LOCUS       MG913180                5267 bp    DNA     linear   INV 17-DEC-2018
DEFINITION  Toxoplasma gondii isolate TgCTBr04 dihydropteroate synthase (dhps)
            gene, complete cds.
ACCESSION   MG913180
VERSION     MG913180.1
KEYWORDS    .
SOURCE      Toxoplasma gondii
  ORGANISM  Toxoplasma gondii
            Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
            Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Silva,L.A., Machado,A.S., Fernandes,M.D. and Vitor,R.W.A.
  TITLE     Efficacy of sulfadiazine and pyrimetamine in the treatment of
            experimental toxoplasmosis with strains obtained from human cases
            of congenital disease in Brazil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Silva,L.A., Machado,A.S., Fernandes,M.D. and Vitor,R.W.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2018) Parasitology, Universidade Federal de Minas
            Gerais, Av. Antonio Carlos, 6627 - Pampulha, Belo Horizonte, Minas
            Gerais 31270-901, Brazil
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Clustal Omega v. 1.2.1
            Sequencing Technology :: Macrogen
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Toxoplasma gondii"
                     /mol_type="genomic DNA"
                     /isolate="TgCTBr04"
                     /isolation_source="newborn"
                     /host="Homo sapiens"
                     /specimen_voucher="UFMG 014"
                     /db_xref="taxon:5811"
                     /country="Brazil"
                     /lat_lon="19.44 S 45.15 W"
                     /collection_date="31-May-2007"
                     /collected_by="Carneiro et al."
                     /note="genotype: ToxoDB#108"
     gene            <91..>5094
                     /gene="dhps"
     mRNA            join(<91..1725,2490..2619,2837..2915,3407..3544,
                     4223..4379,4987..>5094)
                     /gene="dhps"
                     /product="dihydropteroate synthase"
     CDS             join(91..1725,2490..2619,2837..2915,3407..3544,4223..4379,
                     4987..5094)
                     /gene="dhps"
                     /EC_number="2.5.1.15"
                     /codon_start=1
                     /product="dihydropteroate synthase"
                     /protein_id="AZL87737.1"
                     /translation="MGCWPPSFACLRLSWWNLPLFHKRSASFFWPPPGLRLRSLPGGA
                     IAHCASSRSFSFGLLQSKFSRFFTRFHQGTMTANSHFLQKMREEDPGKEYATAYIALG
                     SNLQGDARLGIIEEAISELGRCLGPILGTSCLYETVPAFDVCPKGVVHDVFHPFYLNA
                     VVKLRTPITDPWYVLEILKNLEAKTGRPLPTPEDMEAALNDCTSSGEATNEDVPGQGN
                     KGASRKGDWKEALKKDKYVRGAPRLLDLDLLFFDKNGRSVVIHPDVHPVDKQWPLTLP
                     HPRIITRNFVLFPLCDLDPEYIHPVEGMSVKELLRINLERRRIHLLNAAEEARQTQEL
                     AAKTNSETSGDHMACSHRCAPPASLHFVHNYRIDGSLAIPRRCFAATTEELWTLGGDA
                     GIVDTLLYIEDVKTHADQIIKVGGEEALSEVQRTQLLRLQRLEKTLREEHPLKLMGIL
                     NVSPDSFTDHFSASVDEAVAAAEAMVTDGADVVDVGGEATNPFRVAGEVPLAVERERV
                     VPVVQKILDKLGNRVIISVDTMKAEVARDAVTAGADWVNDQTGESRKGGEGDPLSFVV
                     GNSTTVVLMHKRGTPDTFDGYQDYDDVVHEVGSWLASMSEALQRGGVGRWRILADPGL
                     GISKNPEQSFELVRGVQRIRQMLPTGIPQLLGFSRKRLVGWGVGESAQDRKGPPTTAT
                     VEGRRWGGAAIAAWCAANTDAVTVVRTHDVKDTRLVRDVTERIQGVKSTINLMEKEPD
                     MAPLYSFW"
     gap             1759..2235
                     /estimated_length=477
     gap             3086..3285
                     /estimated_length=200
     gap             3800..4176
                     /estimated_length=377
     gap             4537..4892
                     /estimated_length=356
ORIGIN      
        1 tcttctgctt ccgcggttat ttgggttgcc actggcgctg ttgttgtcgt ctctgcggtg
       61 tcgccgcgac gttctgtctt cgccacgtga atgggctgct ggccaccatc ttttgcctgt
      121 ttgcggctct cttggtggaa tttgccgctg tttcataaaa ggtctgcgtc ttttttctgg
      181 cctccgcctg ggctgcgtct tcgctctctg cctggaggag caattgcaca ctgtgcgagc
      241 tcgcgttctt tttcctttgg ccttctccag tcaaaatttt cccgtttctt caccagattc
      301 caccaaggaa caatgacggc aaattcacat ttcctgcaga agatgaggga agaagaccct
      361 ggcaaagaat acgcgacagc atacatcgca ttgggatcca acttgcaggg ggatgctcga
      421 ctaggcatca ttgaagaagc gatatccgag ttggggcggt gtctaggacc cattctgggc
      481 acatcgtgcc tgtacgaaac cgttccagcc ttcgacgttt gtccgaaagg cgtcgtgcac
      541 gacgtcttcc atccctttta cctgaatgcc gtcgtgaaac tccgcacccc cattacggat
      601 ccctggtacg tgttggaaat tcttaaaaat ctagaggcga aaacggggcg cccactgcca
      661 actcctgaag acatggaggc ggcgctgaac gactgcacct cctccggaga ggctaccaat
      721 gaggacgtgc ctgggcaagg gaacaaggga gcttcgcgga agggcgactg gaaagaagcg
      781 ctcaaaaaag acaagtacgt ccgcggtgct ccccggctcc ttgaccttga tttgcttttc
      841 tttgacaaga acggaagatc cgttgttata caccctgatg tgcatcccgt cgacaaacag
      901 tggcctctga cactgccgca tccgcgaatt atcacgcgaa atttcgtcct gttccctctg
      961 tgcgacctcg atccggagta catacacccc gtggagggaa tgtctgtaaa ggagctgctt
     1021 cgcatcaacc tggagcggcg ccgcattcat ttgctgaatg ccgcggaaga agcccggcaa
     1081 acacaagaat tggcggcaaa gacgaatagc gagacctccg gtgaccacat ggcgtgctcg
     1141 caccgttgcg caccgccggc atcccttcac tttgtgcaca actacagaat tgacggttcg
     1201 ctggccattc cgcggcggtg tttcgcagcg acgactgaag agctctggac tcttggtggc
     1261 gacgccggca tcgtagatac cctcttgtac attgaagatg tcaagacaca cgcggatcag
     1321 ataatcaagg ttggtggcga ggaagcgctg tcggaggtgc agcggacgca gctgctccgt
     1381 ctgcaacgcc tggagaagac cctgcgtgag gaacatccgc tgaagctcat ggggattctg
     1441 aacgtcagtc ccgactcgtt caccgatcac ttttctgcga gcgtcgacga ggctgttgcc
     1501 gctgcagagg ccatggtaac tgacggcgca gacgtggtcg acgtcggagg cgaggccacg
     1561 aaccctttca gggtggccgg cgaagttccg cttgcagtcg aaagggaacg ggttgttccc
     1621 gttgtgcaaa agatcttgga caaactcggc aaccgcgtca tcatttccgt tgacaccatg
     1681 aaggccgaag tcgcgagaga tgctgtcacc gccggagcgg actgggtaag cctagcgacg
     1741 taatggtcgt acagagat                                              
          [gap 477 bp]    Expand Ns
     2236                 aaaga tggtgcaggc tcgggaaatt tctggtttta tacataaaag
     2281 ctccgcatcc attacagtgc tcgtggaagg gtcacgtgtg cggtgtccgg cctatcctca
     2341 gagcgtgtcc tgttgtcggg actggtgaga acgcgagggg ttctaacgtg aatgagatag
     2401 gggtcgagcc agataaagaa atacgtgttt gcgaaaagtg gcttattacc ttttagcgag
     2461 cgtgagctga ggcatcccgc tgccctcagg tgaacgacca gaccggagag agcaggaagg
     2521 gaggggaagg agacccgctt tccttcgttg ttggcaactc gaccaccgtc gtgctgatgc
     2581 acaaacgcgg cactcccgac acgtttgacg gttatcaagg taggcgtgga aaaactgcag
     2641 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnagttttag ctattgtttc gtggaaagaa catgcaatac ccgtctggat
     2761 cctgaccgtg ctaaaaacga gctcatgatt gcccgttcca tcacttttga acacactttt
     2821 gtgcctgcgt ctccagacta cgacgacgtg gtacacgagg tcggttcgtg gttggccagc
     2881 atgagcgagg ctcttcagcg aggcggcgtc ggcaggtagc tgatgttctt tcccaagctt
     2941 caggagtgtg gagagaaatg cgtattccca ggaggaagct gcgttggcga gatgccgtga
     3001 tagacttagt gctggaagat gacatgcaat gaaattcgtc tttacgcatt ttgataagct
     3061 gtctgtcaac tcgagtagcc tccct                                      
          [gap 200 bp]    Expand Ns
     3286                                                  agtgc ttgcggcttc
     3301 actgaaaacg aggtatgtct ggaggatttg gttaaactct cctggaaaca catatgcttt
     3361 tggtagaaac tggtgtcgtt tccgactttg gcgctatgtt ggataggtgg cggattctcg
     3421 cggatcccgg cttgggaatc tccaagaatc ctgagcagag cttcgagctg gtacggggcg
     3481 ttcagcgcat caggcagatg ttgccgacag gcattcccca gcttctcggc ttctccagaa
     3541 aaaggtatgt cacacaaaag agatgttgtc attgcctcct aacggcgggc gcgaggtcgt
     3601 ttaaggaact gaacgacgaa cattgcagtg gcgctgatag gtgccacgct aaacgcacag
     3661 gttcctgctg aaactgctta gattgcatgt taccaaatgt atctctgcag cagtgtggat
     3721 atgccggatg tttcgtgtag taggcaggtt caacagggtg gcctttctcc atcgctctcc
     3781 aggctcttct gtcgcgtcc                                             
          [gap 377 bp]    Expand Ns
     4177                                        gttg ttggacccca aaaggcgttg
     4201 atttgctgcg ttcatttcac agattggtcg gttggggtgt cggtgaatcc gcgcaagata
     4261 ggaaaggtcc cccgacgact gcaaccgtcg aaggtcgtcg atggggagga gcagcaattg
     4321 ctgcctggtg tgctgcaaac acagacgcgg tgaccgtcgt ccggacgcat gatgtgaagg
     4381 taagcgcact gctcgatgaa agagaacaag tacaagtgga caacgttcgt gctaaggagc
     4441 gcattagtat atccagcgtt acgggcacgt tttcagcctg aatggaggac cgatgtactt
     4501 gagaagctgg catgctcaat catttatgat gcgctc                          
          [gap 356 bp]    Expand Ns
     4893                                    gatgtgca actcatcacc tgagcacctt
     4921 ctgtctttta tgtatgcgtg gacaatgata gttatgggcc tcgattttct atggctttgt
     4981 gtccaggaca ctcggctcgt ccgcgacgtg actgagagga ttcaaggagt gaaaagcacg
     5041 attaacctga tggagaaaga gccagatatg gctccgttat acagcttttg gtgatactca
     5101 gcgaatctcg ttcacgtagt aaacgaggct gatttctgtt gcgtgtgttg ttttcttata
     5161 gagggagtct tgaccattgt cttacttgca aacggtagtt gaactagcac aatttttttc
     5221 atgtccaaga gcgcatcgag ttgcttctgg tggagtcttg gcgagtc
//